How do you store beta-mercaptoethanol?

Storage and Special Handling Store in a tightly closed container in a cool, dry area that is well ventilated, such as a vented cabinet. Do not store on shelf. Store in a secondary container by itself. Compounds incompatible with Beta Mercaptoethanol are oxidizers, acids, metals, and ignition sources.

Does beta-mercaptoethanol need to be refrigerated?

At a previous lab, our beta-mercap was kept on the shelf at RT and we never had an issue with it, at least during the 4 years I worked there. But it is recommended to store at 4°C. For a dilution, definitely store it at 4°C. I’ve read about people freezing it at -20°C, which is fine for long-term storage.

Why is B mercaptoethanol added to the SDS-PAGE?

Why do we need add beta-mercaptoethanol in sample buffer to determine Bromelain’s MW in SDS-PAGE? The role of beta-mercaptoethanol is to break all the disulfide bonds and denature the protein of interest.

What is beta-mercaptoethanol used for?

Beta-mercaptoethanol (ß-ME) is a reducing agent that will irreversibly denature RNases by reducing disulfide bonds and destroying the native conformation required for enzyme functionality.

How long is beta mercaptoethanol stable?

1 month
Beta-Mercaptoethanol (ß-ME) is stable for 1 month, but Buffer RLT itself is stable for at least 9 months at room temperature (15 to 25°C).

Does mercaptoethanol expire?

The product is manufactured in a batch process. 5.2 Expiration date and/or recommended re-evaluation interval: 36 months from manufacturing date • The recommended retest period is based on historical data from Sigma-Aldrich’s catalog product grade.

How do I get rid of beta mercaptoethanol smell?

BME odor can be neutralized using standard household bleach. Bleach acts as an oxidizer and converts the thiol group of beta mercaptoethanol into a sulfonic acid derivative which eliminates the natural gas odor. Be sure to absorb any excess BME liquid with an inert absorbent prior to odor decontamination with bleach.

Is mercaptoethanol beta stable?

Gibco® 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. Mercaptoethanol is not stable in solution so most protocols require daily supplementation.

Why does SDS-PAGE have two pH?

Gel Layers: It Takes Two The top (stacking) layer has a lower percentage of acrylamide and a lower pH (6.8) than the bottom (resolving) layer, which has more acrylamide and a higher pH (8.8). SDS PAGE is run in a discontinuous buffer system. The running buffer has different ions and a different pH than the gels.

Can I use BME instead of DTT?

When preparing SDS-PAGE sample buffer, you can use either beta-mercaptoethanol (BME) or dithiothreitol (DTT). For BME, use a concentration of 5% (about 100 mM). For DTT, use 5-10 mM.

How long is beta mercaptoethanol good for?

Is beta mercaptoethanol toxic?

BME can be toxic if ingested, and fatal if inhaled or absorbed through the skin. Vapors can irritate the eyes, mucous membranes, and respiratory tract. Symptoms of inhalation exposure may include coughing, sore throat, and/or shortness of breath.