What is the assay in HPLC?

In summary, High-performance liquid chromatography (HPLC) Analysis is an analytical chemistry technique to separate, detect, and quantitate different drugs and metabolites within various formulations, vehicles, and biological matrices.

What is HPLC used to test for?

High-performance liquid chromatography or high-pressure liquid chromatography (HPLC) is a chromatographic method that is used to separate a mixture of compounds in analytical chemistry and biochemistry so as to identify, quantify or purify the individual components of the mixture.

What is HPLC method?

High-performance liquid chromatography (HPLC) is an important analytical method commonly used to separate and quantify components of liquid samples. In this technique, a solution (first phase) is pumped through a column that contains a packing of small porous particles with a second phase bound to the surface.

How many types of HPLC are there?

HPLC analyses fall into four different categories: (1) reverse phase chromatography where the stationary phase is hydrophobic (C18-modified silica), (2) normal phase chromatography where the stationary phase is hydrophilic (silica), (3) hydrophilic interaction chromatography, a hybrid of techniques 1 and 2, and (4) ion …

How is assay calculated?

The industry-accepted formula for assay on anhydrous basis = (assay on as-is basis×100)/(100-%water). Statistically, the two variables involved in accepted formula are assay on as-is basis and water to obtain assay on anhydrous basis.

What is the basic principle of HPLC?

The separation principle of HPLC is based on the distribution of the analyte (sample) between a mobile phase (eluent) and a stationary phase (packing material of the column). Depending on the chemical structure of the analyte, the molecules are retarded while passing the stationary phase.

What area does HPLC are commonly used?

High-performance liquid chromatography (HPLC) is a chromatographic technique used to split a mixture of compounds in the fields of analytical chemistry, biochemistry and industrial. The main purposes for using HPLC are for identifying, quantifying and purifying the individual components of the mixture.

What is basic principle of HPLC?

How is assay calculated in HPLC?

LOD – Loss On Drying. Assay content by HPLC : Assay = [ At x Ws x P x (100 – LOD of std ) ] / [ As x Wt x ( 100 – LOD of sample) ], P – Potency or Assay of standard.

What are the disadvantages of HPLC?

The disadvantage of HPLC: HPLC can be an expensive method, it required a large number of expensive organics, needs a power supply, and regular maintenance is required. It can be complicated to troubleshoot problems or develop new methods.

HPLC Theory. HPLC works following the basic principle of thin layer chromatography or column chromatography, where it has a stationary phase ( solid like silica gel) and a mobile phase (liquid or gas). The mobile phase flows through the stationary phase and carries the components of the mixture with it.

What is the difference between HPLC and HPTLC?

HPLC is high-pressure liquid chromatography or high-performance liquid chromatography while HPTLC is high-performance thin-layer chromatography. Another major difference between HPLC and HPTLC is that HPLC is a type of column chromatography while HPTLC is a type of planar chromatography.

What is the HPLC used for?

Pharmaceutical HPLC Equipment HPLC (known as high performance liquid chromatography, high pressure liquid chromatography) is used to separate the phases of a solid or liquid analyte, regardless of its stability and volatility. HPLC equipment is popular for its regarded ease of set up, use, and configuration potential.